Improved pretreatment of yellow poplar biomass using hot compressed water and enzymatically-generated peracetic acid. (October 2017)

• Record Type: Journal Article
• Authors: Lee, Hyeong Rae; Lee, Hun Wook; Lee, Youn-Woo; Kazlauskas, Romas J.; Park, Tai Hyun
• Is Part Of: Biomass and bioenergy. Volume 105(2017:Oct.)
• Page Start: 190
• Page End: 196
• Publication Date: 2017-10
• Subjects: Yellow poplar -- Hot compressed water -- Peracetic acid -- Pretreatment -- Cellulase accessibility; Biomass energy -- Periodicals; Biomass -- Periodicals; Energy-Generating Resources -- Periodicals; Bioénergie -- Périodiques; 333.9539
• DOI: 10.1016/j.biombioe.2017.07.004
• Languages: English
• ISSNs: 0961-9534

Abstract

Abstract: Biomass forms a complex interwoven structure containing cellulose, hemicellulose and lignin that hinders enzymatic hydrolysis of cellulose. Enzymatic hydrolysis of the cellulose within yellow poplar (tulip tree) particles released only 9% of the total glucose in this study. To increase the accessibility of the cellulose component, wood particles were pretreated using hot compressed water and enzymatically-generated peracetic acid. The combined pretreatment started with hot compressed water (200 °C, 15 min), which selectively solubilized up to 90% of the xylan. The remaining solid was treated with peracetic acid (90 mM, 60 °C, 6 h), which solubilized up to 70% of the lignin. The remaining solid consisted of mainly glucan (∼75%) and corresponds to 87% of the glucan initially present in the yellow poplar particles. Hydrolysis of the remaining solid using a low loading of cellulase/β-glucosidase for 72 h released 90% of the glucose. The removal of the xylan and lignin structural barriers dramatically increased the cellulase accessibility to cellulose. The structural characteristics (crystallinity, functional group changes, morphology) of combined pretreated solid residue changed in a manner consistent with increased enzymatic digestibility. The combined pretreatment with hot compressed water and peracetic acid was more effective than either single pretreatment and more effective than the sum of the single pretreatments to remove xylan and lignin, thus demonstrating a cooperative effect of the two pretreatments. In addition, the combined pretreatment enhanced the accessibility of cellulases to the cellulose resulting in more efficient cellulose hydrolysis. Highlights: Hot compressed water pretreatment of yellow poplar hydrolyzed xylan to xylose. Subsequent peracetic acid treatment solubilized lignin leaving a glucan-rich solid. Combined pretreatment removes more lignin than the sum of individual pretreatments. Combined pretreatment could enhance enzymatic digestibility and reduce the amount of cellulase needed. This fractionation of the major components of biomass allows separate uses for each component.