Attenuation of the cyproterone acetate‐induced testicular hypofunction by a novel nutraceutical lycopene: a genomic approach. (24th November 2016)

• Record Type: Journal Article
• Authors: Tripathy, A.; Ghosh, A.; Dey, A.; Pakhira, B. P.; Ghosh, D.
• Is Part Of: Andrologia. Volume 49:Number 8(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-11-24
• Subjects: cyproterone acetate -- lycopene -- male infertility -- oxidative stress -- testicular hypofunction; Andrology -- Periodicals; Infertility, Male -- Periodicals; 616.69
• DOI: 10.1111/and.12709
• Languages: English
• ISSNs: 0303-4569

Abstract

Summary: This study was designed to explore the cyproterone acetate (CPA)‐induced andrological hypofunction and its correction by oral administration of lycopene. In this concern, spermatogenic, biochemical, histological and genomic profiles were studied. Cyproterone acetate administration for 1 month helped to develop infertile model rats. A significant recovery was noted in sperm motility, sperm count, sperm viability, hypo‐osmotic swelling tail‐coiled spermatozoa; activities of testicular ∆ 5, 3β‐hydroxysteroid dehydrogenase (HSD), 17β‐HSD, catalase (CAT) and superoxide dismutase (SOD); and levels of conjugated diene (CD), malondialdehyde (MDA), testicular cholesterol and serum testosterone after the administration of lycopene at 1.5 mg/0.5 ml Tween‐80/100 g body weight/day for last 1 month to infertile model rats. Simultaneously, qRT‐PCR study of Bax, Bcl‐2, caspase‐3, ∆ 5, 3β‐HSD and 17β‐HSD genes in testicular tissue showed a significant rectification towards the control in CPA‐pre‐treated cum CPA–lycopene‐cotreated rats. Side‐by‐side histological and histometric studies showed a significant correction in qualitative analysis of spermatogenesis and seminiferous tubular diameter (STD) in CPA‐pre‐treated cum CPA–lycopene‐cotreated rats. Lycopene showed outstanding efficacy in the management of CPA‐induced testicular hypofunction with special reference to correction in oxidative stress‐induced testicular apoptosis at genomic level.