Evolution of the plasma proteome of divers before and after a single SCUBA dive. Issue 9 (12th May 2017)

• Record Type: Journal Article
• Authors: Lautridou, Jacky; Pichereau, Vianney; Artigaud, Sébastien; Bernay, Benoit; Barak, Otto; Hoiland, Ryan; Lovering, Andrew T.; Eftedal, Ingrid; Dujic, Zeljko; Guerrero, François
• Is Part Of: Proteomics. Volume 11:Issue 9/10(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-05-12
• Subjects: Decompression sickness -- Human -- Plasma -- Proteome -- Proteomics -- SCUBA; Proteomics -- Periodicals; 572.605
• DOI: 10.1002/prca.201700016
• Languages: English
• ISSNs: 1862-8346

Abstract

Abstract : Purpose: Decompression sickness (DCS) is a poorly understood and complex systemic disease caused by inadequate desaturation following a reduction of ambient pressure. A previous proteomic study of ours showed that DCS occurrence but not diving was associated with changes in the plasma proteome in rats, including a dramatic decrease of abundance of the tetrameric form of Transthyretin (TTR). The present study aims to assess the impact on the human blood proteome of a dive inducing significant decompression stress but without inducing DCS symptoms. Experimental design: Twelve healthy male divers were subjected to a single dive at a depth of 18 m of sea water (msw) with a 47‐min bottom time followed by a direct ascent to the surface at a rate of 9 msw/min. Venous blood was collected before the dive as well as 30 min and 2 h following the dive. The plasma proteomes from four individuals were then analyzed by using a two‐dimensional electrophoresis‐based proteomic strategy. Results: No protein spot showed a significantly changed abundance (fdr< 0.1) between the tested times. Conclusion: These results strengthen the hypothesis according to which significant changes of the plasma proteome measurable with two‐dimensional electrophoresis may only occur along with DCS symptoms.