Photoactivatable Mussel‐Based Underwater Adhesive Proteins by an Expanded Genetic Code. (1st August 2017)

• Record Type: Journal Article
• Authors: Hauf, Matthias; Richter, Florian; Schneider, Tobias; Faidt, Thomas; Martins, Berta M.; Baumann, Tobias; Durkin, Patrick; Dobbek, Holger; Jacobs, Karin; Möglich, Andreas; Budisa, Nediljko
• Is Part Of: Chembiochem. Volume 18:Number 18(2017)
• Page Start: 1819
• Page End: 1823
• Publication Date: 2017-08-01
• Subjects: biomimetic synthesis -- computational chemistry -- mussel adhesive protein -- photochemistry -- scanning probe microscopy -- synthetases; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201700327
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract: Marine mussels exhibit potent underwater adhesion abilities under hostile conditions by employing 3, 4‐dihydroxyphenylalanine (DOPA)‐rich mussel adhesive proteins (MAPs). However, their recombinant production is a major biotechnological challenge. Herein, a novel strategy based on genetic code expansion has been developed by engineering efficient aminoacyl‐transfer RNA synthetases (aaRSs) for the photocaged noncanonical amino acid ortho ‐nitrobenzyl DOPA (ONB‐DOPA). The engineered ONB‐DOPARS enables in vivo production of MAP type 5 site‐specifically equipped with multiple instances of ONB‐DOPA to yield photocaged, spatiotemporally controlled underwater adhesives. Upon exposure to UV light, these proteins feature elevated wet adhesion properties. This concept offers new perspectives for the production of recombinant bioadhesives. Abstract : Light‐controllable biomimetic glue : Expansion of the genetic code with the noncanonical amino acid (ncAA) ortho ‐nitrobenzyl 3, 4‐dihydroxyphenylalanine (ONB‐DOPA) allows the production of photoactivatable mussel adhesive proteins in vivo. Carrying the ncAA at multiple sites, these proteins show spatiotemporally controllable adhesive properties.