Development of an LC–MS/MS method for quantification of two pairs of isomeric flavonoid glycosides and other ones in rat plasma: Application to pharmacokinetic studies. (16th April 2017)

• Record Type: Journal Article
• Authors: Zhang, Sixi; Xie, Yang; Wang, Jing; Geng, Yanmei; Zhou, Yu; Sun, Chengxin; Wang, Guangshu
• Is Part Of: Biomedical chromatography. Volume 31:Number 10(2017:Oct.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-04-16
• Subjects: flavonoid glycosides -- LC–MS/MS -- pharmacokinetic studies -- rat plasma -- Scorzonera Austriaca; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3972
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: A liquid chromatography–tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous determination of six flavonoid glycosides – isoorientin (1 ), orientin (2 ), 2″‐ O‐β ‐d ‐xylopyranosyl isoorientin (3 ), 2″‐ O‐β ‐d ‐xylopyranosyl isovitexin (4 ), 6‐C‐l ‐ α ‐arabipyranosyl vitexin (5 ) and vitexin (6 ) – in rat plasma using isoquercitrin as the internal standard (IS). Plasma samples were prepared by a one‐step protein precipitation with acetonitrile. Chromatographic analysis was carried out on a 25 cm C18 column with a gradient mobile phase consisting of acetonitrile and 0.1% aqueous formic acid. Six analytes and IS were detected through electrospray ionization in negative‐ion selection reaction monitoring mode. The mass transitions were as follows: m/z 447.2 → 327.0 for1, m/z 447.2 → 327.0 for2, m/z 579.3 → 458.9 for3, m/z 563.0 → 293.1 for4, m/z 563.0 → 353.0 for5, m/z 431.1 → 311.1 for6, and m/z 463.1 → 300.2 for IS. Calibration curves exhibited good linearity ( r 2 > 0.9908) over a wide concentration range for all compounds. Intra‐ and inter‐day precision (RSD, %) at four different levels were both <14.2% and the accuracy (RE, %) ranged from −11.9 to 12.0%. The extraction recoveries of the six components ranged from 88.2 to 103.6%. The validated assay was successfully applied to the pharmacokinetic studies of the six components in male rat plasma after intravenous administration of total flavonoids of Scorzonera austriaca Wild.