An enzymatic strategy to asymmetrically branched N-glycans. Issue 35 (23rd August 2017)

• Record Type: Journal Article
• Authors: Calderon, Angie D.; Zhou, Jun; Guan, Wanyi; Wu, Zhigang; Guo, Yuxi; Bai, Jing; Li, Qing; Wang, Peng George; Fang, Junqiang; Li, Lei
• Is Part Of: Organic & biomolecular chemistry. Volume 15:Issue 35(2017)
• Page Start: 7258
• Page End: 7262
• Publication Date: 2017-08-23
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c7ob01765k
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Using a set of glycosidases and glycosyltransferases, an enzymatic strategy was developed to prepare asymmetric N -glycans excluding any chemical procedures. Abstract : An enzymatic strategy was developed to generate asymmetrically branched N -glycans from natural sources by using a panel of glycosidases and glycosyltransferases. Briefly, LacZ β-galactosidase was employed to selectively trim symmetrically branched N -glycans isolated from bovine fetuin. The yielding structures were then converted to asymmetrically branched core structures by robust glycosyltransferase for further extension.