Higher platelet reactivity and platelet-monocyte complex formation in Gram-positive sepsis compared to Gram-negative sepsis. (18th August 2017)

• Record Type: Journal Article
• Authors: Tunjungputri, Rahajeng N.; van de Heijden, Wouter; Urbanus, Rolf T.; de Groot, Philip G.; van der Ven, Andre; de Mast, Quirijn
• Is Part Of: Platelets. Volume 28:Number 6(2017)
• Page Start: 595
• Page End: 601
• Publication Date: 2017-08-18
• Subjects: Gram-positive -- gram-negative -- infection -- platelet activation -- sepsis; Blood platelets -- Periodicals; Blood Platelets -- Periodicals; 615.39
• DOI: 10.1080/09537104.2016.1252837
• Languages: English
• ISSNs: 0953-7104

Abstract

Abstract: Platelets may play a role in the high risk for vascular complications in Gram-positive sepsis. We compared the platelet reactivity of 15 patients with Gram-positive sepsis, 17 with Gram-negative sepsis and 20 healthy controls using a whole blood flow cytometry-based assay. Patients with Gram-positive sepsis had the highest median fluorescence intensity (MFI) of the platelet membrane expression of P-selectin upon stimulation with high dose adenosine diphosphate (ADP; P = 0.002 vs. Gram-negative and P = 0.005 vs. control groups) and cross-linked collagen-related peptide (CRP-XL; P = 0.02 vs. Gram-negative and P = 0.0001 vs. control groups). The Gram-positive group also demonstrated significantly higher ADP-induced fibrinogen binding ( P = 0.001), as wll as platelet-monocyte complex formation ( P = 0.02), compared to the Gram-negative group and had the highest plasma levels of platelet factor 4, β-thromboglobulin and soluble P-selectin. In contrast, thrombin-antithrombin complex and C-reactive protein levels were comparable in both patient groups. In conclusion, common Gram-positive pathogens induce platelet hyperreactivity, which may contribute to a higher risk for vascular complications