Bio-catalytic nanocompartments for in situ production of glucose-6-phosphate. Issue 73 (29th August 2017)

• Record Type: Journal Article
• Authors: Lomora, M.; Gunkel-Grabole, G.; Mantri, S.; Palivan, C. G.
• Is Part Of: Chemical communications. Volume 53:Issue 73(2017)
• Page Start: 10148
• Page End: 10151
• Publication Date: 2017-08-29
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc04739h
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Bio-catalytic phosphoglucomutase based polymer nanocompartments permeabilized with engineered α-hemolysin for the production of glucose-6-phosphate needed to aid in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions. Abstract : Cells are sophisticated biocatalytic systems driving a complex network of biochemical reactions. A bioinspired strategy to create advanced functional systems is to design confined spaces for complex enzymatic reactions by using a combination of synthetic polymer assemblies and natural cell components. Here, we developed bio-catalytic nanocompartments that contain phosphoglucomutase protected by a biomimetic polymer membrane, which was permeabilized for reactants through insertion of an engineered α-hemolysin pore protein. These bio-catalytic nanocompartments serve for production of glucose-6-phosphate, and thus possess great potential for applications in an incomplete glycolysis, pentose phosphate pathway, or in plant biological reactions.