Polarization sensitive excitation-emission matrices for detection of colorectal tumours – initial investigations. (18th March 2015)

• Record Type: Journal Article
• Authors: Borisova, E; Genova, Ts; Zhelyazkova, Al; Angelova, L; Keremedchiev, M; Penkov, N; Vladimirov, B; Semyachkina-Glushkovskaya, O; Avramov, L
• Is Part Of: Journal of physics. Number 594 (2015)
• Page Start: 012031
• Publication Date: 2015-03-18
• Subjects: Physics -- Congresses; 530.5
• DOI: 10.1088/1742-6596/594/1/012031
• Languages: English
• ISSNs: 1742-6588

Abstract

Abstract: Excitation-emission matrices were used for detection of the autofluorescence properties of surgically excised normal and cancerous mucosa of lower gastrointestinal tract tissues (colon and rectum carcinoma). Linear polarization of the excitation and emission fluorescence light was additionally applied to evaluate the influence of anisotropic fluorophores presented in the tissues investigated. Excitation applied was in the region of 280-440 nm, using step of 10 nm for the scanning, fluorescence emission was detected in the region of 300-800 nm, with scanning step of 1 nm. Excitation and emission light were investigated in parallel and perpendicular linear polarization modes respectively. These investigations are part of the concept to proof the feasibility of autofluorescence system for a real clinical application. Autofluorescence detection could make the entire diagnostics procedure more personal, patient friendly and effective and will help for further understanding of tumors nature and to improve patients' lives. In the current investigation major spectral features without and with linear polarization applied are addressed and lower GIT lesions' emission properties are evaluated.