The analyzation and clinical evaluation of ECLIA and CMIA in the detection of Treponema pallidum. Issue 24 (June 2017)

• Record Type: Journal Article
• Authors: Zhou, Jiansuo; Liang, Yongming; Zhang, Jie; Cui, Liyan
• Other Names: Pany. Satyabrata section editor.
• Is Part Of: Medicine. Volume 96:Issue 24(2017)
• Publication Date: 2017-06
• Subjects: chemiluminescent magnetic microparticle immunoassay -- electrochemiluminescence immunoassay analyzer -- recombinant immunoblot assay -- Treponema pallidum -- Treponema pallidum particle agglutination test; Medicine -- Periodicals; Medicine -- Periodicals; Médecine -- Périodiques; Geneeskunde; Medicine; Periodicals; Periodicals; 610.5
• DOI: 10.1097/MD.0000000000007139
• Languages: English
• ISSNs: 0025-7974

Abstract

Abstract : Abstract: To evaluate the clinical application value of electrochemiluminescence immunoassay analyzer (ECLIA) and chemiluminescent magnetic microparticle immunoassay (CMIA) in the detection of Treponema pallidum (TP). A total of 1225 patients in Peking University Third Hospital was enrolled from June 2014 to October 2014. ECLIA and CMIA were applied to detect the serum anti-TP. The positive rate was analyzed. RIBA was taken as a golden standard to evaluate the sensitivity, the specificity, the positive predictive value, the negative predictive value, and the accuracy of ECLIA and CMIA. A correlation analysis between 2 assays was conducted, and that between assay and RIBA. We also evaluate the clinical value of TPPA in the detection of T pallidum . The positive rate of CMIA and ECLIA is 10.63% and 9.89%, respectively, showing no statistically significant difference ( P > .05). For CMIA, ECLIA, and TPPA, the sensitivity is 99.16%, 99.16%, and 99.16%, the specificity is 98.99%, 99.82%, and 100%, the positive predictive value is 91.47%, 98.33%, and 100%, the negative predictive value is 99.91%, 99.91%, and 99.91%, the coincidence rate is 99.01% (Kappa = 0.895), 99.75% (Kappa = 0.997), and 99.92% (Kappa = 0.998), respectively. The result shows high correlation between ECLIA and CMIA. Both have high sensitivity and specificity and can be used as screening tests for the diagnosis of T pallidum in common condition.