Effect of deletion of 2, 3-butanediol dehydrogenase gene (bdhA) on acetoin production of Bacillus subtilis. Issue 8 (14th September 2017)

• Record Type: Journal Article
• Authors: Zhang, Junjiao; Zhao, Xiangying; Zhang, Jiaxiang; Zhao, Chen; Liu, Jianjun; Tian, Yanjun; Yang, Liping
• Is Part Of: Preparative biochemistry & biotechnology. Volume 47:Issue 8(2017)
• Page Start: 761
• Page End: 767
• Publication Date: 2017-09-14
• Subjects: 2, 3-Butanediol -- 2, 3-butanediol dehydrogenase gene (bdhA) -- acetoin -- Bacillus subtilis -- deletion -- transformation; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2017.1320293
• Languages: English
• ISSNs: 1082-6068

Abstract

ABSTRACT: The present work aims to block 2, 3-butanediol synthesis in acetoin fermentation of Bacillus subtilis . First, we constructed a recombinant strain BS168D by deleting the 2, 3-butanediol dehydrogenase gene bdhA of the B. subtilis168, and there was almost no 2, 3-butanediol production in 20 g/L of glucose media. The acetoin yield of BS168D reached 6.61 g/L, which was about 1.5 times higher than that of the control B. subtilis168 (4.47 g/L). Then, when the glucose concentration was increased to 100 g/L, the acetoin yield reached 24.6 g/L, but 2.4 g/L of 2, 3-butanediol was detected at the end of fermentation. The analysis of 2, 3-butanediol chiral structure indicated that the main 2, 3-butanediol production of BS168D was meso-2, 3-butanediol, and the bdhA gene was only responsible for (2R, 3R)-2, 3-butanediol synthesis. Therefore, we speculated that there may exit another pathway relating to the meso-2, 3-butanediol synthesis in the B. subtilis . In addition, the results of low oxygen condition fermentation showed that deletion of bdhA gene successfully blocked the reversible transformation between acetoin and 2, 3-butanediol and eliminated the effect of dissolved oxygen on the transformation.