Fabrication of highly stable and sensitive electrochemical sensor from hemoglobin–Au nanocomposites and its analytical applications. Issue 68 (5th September 2017)

• Record Type: Journal Article
• Authors: Wu, Weiping; Fang, Yuting; Zhu, Chenchen; Chen, Shiyu; Li, Ting; Wu, Lei; Bao, Ning; Liu, Yang; Gu, Haiying
• Is Part Of: RSC advances. Volume 7:Issue 68(2017)
• Page Start: 42884
• Page End: 42890
• Publication Date: 2017-09-05
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c7ra05808j
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Hemoglobin–Au nanocomposites fabrication of highly stable and sensitive electrochemical sensor. Abstract : To improve electron transfer, a combination of electroactive hemoglobin (Hb) and Au nanoparticles (Au NPs) was designed to obtain nanocomposites (NCs). Cyclic voltammetry and redox probe characterization showed that Hb–Au NCs incorporating the layer-by-layer technique to improve the ration of electroactive Hb, showed a better conjugated structure, lower standard entropy, and faster electron transfer. Au entrapped in Hb on the glassy carbon electrode (GCE) also exhibited a wider linear range, and excellent reproducibility and recovery for analytical applications of hydrogen peroxide, oxygen, and trichloroacetic acid. Therefore, our research could potentially become a promising alternative by combining bioactive molecules with nanoparticles, and broaden the applications of mediator-free biosensors.