Dehydration of blood platelets by zeodration: in vitro characterization and hemostatic properties in vivo. Issue 9 (9th April 2015)

• Record Type: Journal Article
• Authors: Donnet, Thibault; Ravanat, Catherine; Eckly, Anita; Maurer, Eric; Alame, Ghina; Ziessel, Catherine; Mangin, Pierre H.; Freund, Monique; Cazenave, Jean‐Pierre; Gachet, Christian; Rendu, Francine
• Is Part Of: Transfusion. Volume 55:Issue 9(2015)
• Page Start: 2207
• Page End: 2218
• Publication Date: 2015-04-09
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.13121
• Languages: English
• ISSNs: 0041-1132

Abstract

Abstract : BACKGROUND: Platelets (PLTs) are currently stored at room temperature (RT) for 5 to 7 days. So far, there exists no validated method for the preparation and long‐term storage of dehydrated PLTs suitable for transfusion after rehydration. In this study, a desiccation process, zeodration, was applied to PLTs. STUDY DESIGN AND METHODS: A complete procedure of dehydration at RT by zeodration was employed. Zeodrated human and mouse PLTs were characterized in vitro. Zeodrated mouse PLTs were transfused into clopidogrel‐treated mice to evaluate their hemostatic properties. RESULTS: The optimal conditions for dehydration of PLTs at RT in a laboratory scale zeodrator were defined as 145 mbar and 20.2 ± 1.5°C. The recovery rate was 85 ± 2% and the dryness of zeodrated PLTs (Z_PLTs) indicated that they were sufficiently stable for long‐term storage. Rehydrated Z_PLTs were round, were not aggregated, and expressed the glycoproteins required for PLT function. Z_PLTs agglutinated in the presence of von Willebrand factor (VWF) and aggregated in response to thrombin or collagen independently of an active metabolism. In a flow system, Z_PLTs could adhere to VWF and form aggregates on a collagen matrix. Thrombin was generated at the surface of Z_PLTs as efficiently as on fresh PLTs. In clopidogrel‐treated mice, which exhibited a severely prolonged bleeding time, continuous perfusion of Z_PLTs restored normal hemostasis. CONCLUSION: Zeodration represents a new strategy to prepare PLTs with partly preserved aggregative properties after storage and rehydration. Z_PLTs have potential hemostatic properties provided it is possible to improve their transfusion efficacy.