Determination of cloxacillin residues in dairy cows after intramammary administration. Issue 5 (30th January 2017)

• Record Type: Journal Article
• Authors: Burmańczuk, A.; Grabowski, T.; Osypiuk, M.; Polska, B.; Kowalski, C.
• Is Part Of: Journal of veterinary pharmacology and therapeutics. Volume 40:Issue 5(2017)
• Page Start: 552
• Page End: 560
• Publication Date: 2017-01-30
• Subjects: Veterinary pharmacology -- Periodicals; Therapeutics -- Periodicals; 636.0895105
• DOI: 10.1111/jvp.12391
• Languages: English
• ISSNs: 0140-7783

Abstract

Abstract : The aim of this study was to perform a comparative analysis of the characteristics of cloxacillin (CLO) (MRL of withdrawal in bovine milk is 30 ng/g) after a single intramammary (IMM) dose in the dry period (DP) and lactation (LP), and to establish a high‐performance liquid chromatography (HPLC) analytical method for CLO detection in milk. The research was conducted on a group of 10 cows in DP and 10 in LP. A single dose of 600 mg of CLO was administrated by the IMM route for a single quarter in DP and 500 mg for a single quarter in LP. CLO concentration was analyzed by HPLC. CLO was monitored at a wavelength of 206 nm. Pharmacokinetic calculations were performed using Phoenix ® WinNonlin ® 6.4 software. The calibration curve was linear over the range of 13.03–28 019.00 ng/g with the coefficient of determination R 2 > 0.999. CLO withdrawal in both the LP and DP group had a biphasic nature. The total CLO elimination in the DP and LP group was reached after 36 and 6.5 days, respectively. A quantitative and confirmatory method for the determination of CLO in fresh milk has been established. We have confirmed that the withdrawal of CLO in the DP group is not a linear process and has a stepwise character.