Anaesthetic efficacy of eugenol on various size classes of angelfish (Pterophyllum scalare Schultze, 1823). (26th April 2017)

• Record Type: Journal Article
• Authors: Tarkhani, Reza; Imani, Ahmad; Jamali, Hadi; Farsani, Hamed Ghafari
• Is Part Of: Aquaculture research. Volume 48:Number 10(2017)
• Page Start: 5263
• Page End: 5270
• Publication Date: 2017-04-26
• Subjects: anaesthesia -- behavioural response -- eugenol -- Pterophyllum scalare; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.13339
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: Anaesthetic efficacy of eugenol was investigated on Pterophyllum scalare . A total of 130 fish with average weights of 1.0 ± 0.5, 5.0 ± 1.0 and 10.0 ± 1.0 g were subjected to 1.25, 2.5, 4.0, 5.5 and 7.0 mg/L eugenol, and behavioural responses were observed. Induction and recovery times were significantly affected by the interactive effect of eugenol concentration and fish weight ( p < .05). Generally, 49.9–128 s after exposure to 1.25–7 mg/L eugenol, fish reached stage 3. Fish entered stage 4 over 55–135 s post exposure to such concentrations. Recovery time was 393.5–597.7 s in all sizes. Any increase in eugenol concentration led to a significant decrease in the induction time with a subsequent increment of the recovery time. Concentrations of eugenol and fish size along with their interactive effects have significantly contributed to the regression models, with concentration recording the highest beta values for stages 1, 2, 3 and 4 (−0.903, −0.898, −0.976 and −0.864 respectively) and the product of size and anaesthetic concentration for full recovery in smaller fish (0.647) and eugenol concentration in larger ones (0.967). Recovery time was fitted to induction time to stage 4 via quadratic and linear regression models in smaller and larger fish respectively. Results revealed the minimal eugenol concentration to induce anaesthesia in various size classes of angelfish in less than 3 min was 1.25 mg/L. Our results showed eugenol as an effective and safe anaesthetic; however, it is not advisable for live fish transportation.