The role of Dickkopf-1 as a potential prognostic marker in pancreatic ductal adenocarcinoma. Issue 17 (2nd September 2017)

• Record Type: Journal Article
• Authors: Liu, De-Jun; Xie, Yue-Xia; Liu, Xiao-Xing; Huo, Yan-Miao; Yang, Min-Wei; Fu, Xue-Liang; Liu, Wei; Yang, Jian-Yu; Li, Jiao; Hua, Rong; Liu, Pei-Feng; Sun, Yong-Wei; Zhang, Jun-Feng
• Is Part Of: Cell cycle. Volume 16:Issue 17(2017)
• Page Start: 1622
• Page End: 1629
• Publication Date: 2017-09-02
• Subjects: DKK-1 -- Pancreatic cancer -- Pancreatic ductal adenocarcinoma -- Tissue microarray -- Prognosis -- Biomarker -- Wnt signaling; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2017.1356510
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Dickkopf-1(DKK-1), the downstream target of β-catenin/T-cell factor, participates in a negative feedback loop in the Wnt signaling and reported as an important biomarker in many tumors. In this study, we analyzed the expression of DKK-1 in pancreatic ductal adenocarcinoma (PDAC) patients at both mRNA and protein levels. We used real-time PCR to detect the expression of DKK-1 in 32 PDAC and paired adjacent non-tumor tissues, results suggested that the expression of DKK-1 was increased in PDAC tissues. We found the similar results in the analysis of 3 independent microarray data sets. Immunohistochemical staining of 311 pairs of PDAC tissues suggested that DKK-1 expression was significantly associated with T classification (P = 0.039) and lymph node metastasis (P = 0.035). Furthermore, Kaplan-Meier analysis for DKK-1 expression demonstrated that patients with higher DKK-1 level had shorter overall survival (OS) and relapse-free survival (RFS) time in Ren Ji cohort and online PDAC database at both mRNA and protein levels. Univariable and multivariable Cox regression analysis confirmed that DKK-1 as well as lymph node metastasis and histology were independent predictors of OS in patients with PDAC. This study demonstrated that DKK-1 may be a predictor for prognosis in PDAC patients.