Determination of Immunoglobulin G by a Hemin–Manganese(IV) Oxide-Labeled Enzyme-linked Immunosorbent Assay. (24th July 2017)

• Record Type: Journal Article
• Authors: Guo, Linyan; Qian, Pin; Yang, Minghui
• Is Part Of: Analytical letters. Volume 50:Number 11(2017)
• Page Start: 1803
• Page End: 1811
• Publication Date: 2017-07-24
• Subjects: Enzyme-linked immunosorbent assay -- hemin–manganese(IV) oxide nanocomposite -- human immunoglobulin G -- ultraviolet–visible absorption spectroscopy; Chemistry, Analytic -- Periodicals; Chemistry, Analytic -- Abstracts; 543
• DOI: 10.1080/00032719.2016.1251447
• Languages: English
• ISSNs: 0003-2719

Abstract

ABSTRACT: An enzyme-linked immunosorbent assay (ELISA) is reported for human immunoglobulin G based on synthesized hemin–MnO2 nanocomposite as the label. Enhanced sensitivity was obtained due to the increased catalytic activity of the hemin–MnO2 nanocomposite toward 3, 3′, 5, 5′-tetramethylbenzidine compared to hemin and MnO2 alone. The synthesized hemin–MnO2 nanocomposite was characterized by transmission electron microscopy and its catalytic activity to 3, 3′, 5, 5′-tetramethylbenzidine was investigated by ultraviolet–visible absorption spectroscopy. After assembly of the sandwich-type immunoassay in the 96 wells of the plate the hemin–MnO2 -based label catalyzed 3, 3′, 5, 5′-tetramethylbenzidine into blue compounds that were monitored by a plate reader. The absorbance increased with the concentration of human immunoglobulin G. The immunoassay displayed high sensitivity, a long linear dynamic range, and good selectivity for human immunoglobulin G. The immunoassay was also used for the determination of human immunoglobulin G in serum with favorable results. The developed assay combines the high throughput and low cost of ELISA with the simplicity of nanocomposite labeling and is suitable for application in clinical diagnosis.