A luminescence switch-on assay for the detection of specific gene deletion using G-quadruplex DNA and silver nanoclusters. (17th August 2016)

• Record Type: Journal Article
• Authors: Wang, Modi; Wang, Wanhe; Liu, Chenfu; Liu, Jinbiao; Kang, Tian-Shu; Leung, Chung-Hang; Ma, Dik-Lung
• Is Part Of: Materials chemistry frontiers. Volume 1:Number 1(2017)
• Page Start: 128
• Page End: 131
• Publication Date: 2016-08-17
• Subjects: Materials science -- Periodicals; Chemistry -- Periodicals; 540
• DOI: 10.1039/c6qm00068a
• Languages: English
• ISSNs: 2052-1529

Abstract

Abstract : A switch-on gene deletion detection platform is constructed based on the alteration of the effective distance between G-quadruplex DNA and AgNCs. Abstract : A switch-on gene deletion detection platform is reported based on the alteration of the effective distance between G-quadruplex DNA and silver nanoclusters (AgNCs). Moreover, the role of the G-quadruplex structure in interacting with AgNCs has also been demonstrated. The detection of the deletion mutants of LMP1 and CCR5 genes is performed as the proof-of-concept of our assay. Finally, the potential biological application of the assay was demonstrated in 10% v/v human serum.