Head-to-Head Comparison of Inflammation and Neovascularization in Human Carotid Plaques: Implications for the Imaging of Vulnerable Plaques. (May 2017)

• Record Type: Journal Article
• Authors: Demeure, Fabian; Bouzin, Caroline; Roelants, Véronique; Bol, Anne; Verhelst, Robert; Astarci, Parla; Gerber, Bernhard L.; Pouleur, Anne-Catherine; Pasquet, Agnès; de Meester, Christophe; Vanoverschelde, Jean-Louis J.; Vancraeynest, David
• Is Part Of: Circulation. Volume 10:Number 5(2017)
• Publication Date: 2017-05
• Subjects: contrast ultrasound -- plaque inflammation -- plaque neovascularization -- positron-emission tomography; Cardiovascular system -- Imaging -- Periodicals; Heart -- Imaging -- Periodicals; 616.1075405
• DOI: 10.1161/CIRCIMAGING.116.005846
• Languages: English
• ISSNs: 1941-9651

Abstract

Abstract : Background—: Inflammation and intraplaque neovascularization are acknowledged to be 2 features of plaque vulnerability, although their temporal expression and their respective value in predicting clinical events are poorly understood. To determine their respective temporal associations, we conducted a comprehensive assessment of inflammation and intraplaque neovascularization in the carotid plaque of symptomatic and asymptomatic patients. Methods and Results—: Thirty patients with severe carotid stenosis underwent 18 F-fluorodeoxyglucose-positron emission tomography/computed tomographic imaging. Plaque 18 F-fluorodeoxyglucose-uptake, indicative of inflammation, was measured by calculating the target:background ratio. The presence of intraplaque neovascularization during contrast-enhanced ultrasound was judged semiquantitatively; low-grade contrast enhancement (CE) suggested its absence, and high-grade CE, the presence of neovascularization. Carotid surgery was performed 1.6±1.8 days after completing both imaging modalities in all patients, and the presence of macrophages and neovessels was quantified by immunohistochemistry. We identified a significant correlation between the target:background ratio and macrophage quantification ( R =0.78; P <0.001). The number of vessels was also significantly higher in carotid plaque with high-CE ( P <0.001). Surprisingly, immunohistochemistry showed that high-CE and vessel number were neither associated with an elevated target:background ratio ( P =0.28 and P =0.60, respectively) nor macrophage infiltration ( P =0.59 and P =0.40, respectively). Finally, macrophage infiltration and target:background ratio were higher in the carotid plaque of symptomatic patients ( P =0.021 and P =0.05, respectively), whereas CE grade and the presence of neovessels were not. Conclusions—: Inflammation and intraplaque neovascularization are not systematically associated in carotid plaques, suggesting a temporal separation between the 2 processes. Inflammation seems more pronounced when symptoms are present. These data highlight the challenges that face any imaging strategy designed to assess plaque vulnerability. Abstract : Supplemental Digital Content is available in the text.