Determination of Acetylcysteine in Pharmaceutical Samples by Silicomolybdenum Blue Spectrophotometry. Issue 3 (13th February 2015)

• Record Type: Journal Article
• Authors: Wen, Xin‐Rong; Tu, Chang‐Qing; Wen, Xiao‐Hong
• Is Part Of: Journal of the Chinese Chemical Society. Volume 62:Issue 3(2015:Mar.)
• Page Start: 296
• Page End: 300
• Publication Date: 2015-02-13
• Subjects: Acetylcysteine -- Silicomolybdenum blue -- Spectrophotometry; Chemistry -- Periodicals; Electronic journals; 540.5
• DOI: 10.1002/jccs.201400344
• Languages: English
• ISSNs: 0009-4536

Abstract

Abstract: An accurate and fast spectrophotometric method for the determination of acetylcysteine by silicomolybdenum blue has been established. The various effect factors on the determination of acetylcysteine by silicomolybdenum blue spectrophotometry are investigated in detail. The results show that under the optimum reaction conditions, SiO3 2‐ reacts with Mo7 O24 6‐ to form silicon molybdenum heteropoly acid (H4 Si(Mo3 O10 )4 ). Then H4 Si(Mo3 O10 )4 is reduced by hydrosulfuryl (‐SH) in acetylcysteine to form silicomolybdenum blue (H4 Si(Mo3 O10 )2 (Mo3 O9 )2 ). The absorbance of silicomolybdenum blue is measured at the maximal absorption wavelength of 735 nm, and the content of acetylcysteine can be calculated based on this absorbance. A good linear relationship is obtained between the absorbance of silicomolybdenum blue and the concentration of acetylcysteine in the range of 5.040∼25.20 μg⋅mL ‐1 . The linear regression equation is A = 0.0272 + 21.484C (mg⋅mL ‐1 ), with a linear correlation coefficient of 0.9995. This proposed method has been successfully applied to the determination of acetylcysteine in pharmaceutical samples, and the results agree well with those obtained by pharmacopoeial method. Abstract : According to the proposed procedures, a series of standard solutions of acetylcysteine were prepared. A good linear relationship between absorbance (A) of silicomolybdenum blue and the concentration (C) of acetylcysteine is obtained in the range of 5.040∼25.20μg⋅mL ‐1 (Fig.5).The linear regression equation is A=0.0272+21.484C