Evaluation of fibroblast attachment in root conditioning with Er, Cr:YSGG laser versus EDTA: A SEM study. Issue 4 (18th February 2015)

• Record Type: Journal Article
• Authors: Fekrazad, Reza; Lotfi, Ghogha; Harandi, Mohammad; Ayremlou, Sara; Kalhori, Katayoun A.M.
• Is Part Of: Microscopy research and technique. Volume 78:Issue 4(2015:Apr.)
• Page Start: 317
• Page End: 322
• Publication Date: 2015-02-18
• Subjects: Er, Cr:YSGG laser -- EDTA -- root conditioning -- scanning electron microscopy; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.22477
• Languages: English
• ISSNs: 1059-910X

Abstract

ABSTRACT: The regeneration of periodontal support is a main concern in periodontal therapy. This study aims to investigate the efficacy of Er, Cr:YSGG laser and EDTA based conditioning in attachment of fibroblast on root surfaces. This in vitro study was conducted on 81 root plates (6 mm × 4 mm × 1 mm) prepared from 27 single‐rooted human mature teeth. The samples were divided into three groups: (1) Er, Cr: YSGG laser conditioning with a G6 tip (2.78 µm, 0.75 W, pulse duration of 140 µs, repetition rate of 20 Hz) for 5–7 s; (2) EDTA conditioning (17%, pH: 8) for 1 min; and (3) the control group which were exposed neither to EDTA nor laser. The viability and proliferation rates assessments were performed using MTT assay on days 3 and 5. In addition, the level of cell attachment was studied using scanning electron microscopy. The data indicated Er, Cr:YSGG conditioning increased cell viability by lapse of time (from days 3–5), with significantly better cell attachment compared to the other groups on days 3 and 5 ( P < 0.05). In addition, increasing cell attachment in the EDTA conditioning group compared with the control group was statistically significant on day 5 but not on day 3 ( P < 0.05). In conclusion, Er, Cr:YSGG laser conditioning can promote enhance fibroblast attachment on dentinal root surfaces more than EDTA. Microsc. Res. Tech. 78:317–322, 2015 . © 2015 Wiley Periodicals, Inc.