Characterization of mesenchymal progenitor cell populations from non‐epithelial oral mucosa. (13th October 2014)

• Record Type: Journal Article
• Authors: Matsumura, S; Higa, K; Igarashi, T; Takaichi, S; Tonogi, M; Shinozaki, N; Shimazaki, J; Yamane, G‐y
• Is Part Of: Oral diseases. Volume 21:Number 3(2015:Apr.)
• Page Start: 361
• Page End: 372
• Publication Date: 2014-10-13
• Subjects: oral mucosa -- mesenchymal stem cells -- differentiation -- neural crest -- regeneration -- corneal stroma; Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.12288
• Languages: English
• ISSNs: 1354-523X

Abstract

Abstract : Objectives: The characteristics of cell populations extracted from oral mucosal non‐epithelial tissues and their ability to differentiate were evaluated in vitro as a potential source of cells for mandibular and corneal regeneration. Materials and methods: Oral mucosal non‐epithelial cells (OMNECs) were extracted from tissue samples and were studied by flow cytometry and RT‐PCR. Cells differentiating into osteoblasts, adipocytes, chondrocytes, neurocytes, or keratocytes were characterized by RT‐PCR and cell staining. Results: OMNECs expressed CD44, CD90, CD105, CD166, and STRO‐1 antigens, which are markers for mesenchymal stem cells. In addition, Oct3/4, c‐Myc, Nanog, KLF4, and Rex, which are expressed by embryonic or pluripotent stem cells, were detected by RT‐PCR. Expression of CD49d, CD56, and PDGFR α, proteins closely associated with the neural crest, was observed in OMNECs, as was expression of Twist1, Sox9, Snail1 and Snail2, which are early neural crest and neural markers. Specific differentiation markers were expressed in OMNECs after differentiation into osteoblasts, adipocytes, chondrocytes, or keratocytes. Conclusions: Populations of OMNECs may contain both mesenchymal stem cells and neural crest origin cells and are a potential cell source for autologous regeneration of mandibular or corneal stroma.