Screening mutations in drug-resistant Mycobacterium tuberculosis strains in Yunnan, China. Issue 5 (September 2017)

• Record Type: Journal Article
• Authors: Li, Daoqun; Song, Yuzhu; Zhang, Cheng-Lin; Li, Xiaofei; Xia, Xueshan; Zhang, A-Mei
• Is Part Of: Journal of infection and public health. Volume 10:Issue 5(2017)
• Page Start: 630
• Page End: 636
• Publication Date: 2017-09
• Subjects: Mycobacterium tuberculosis -- Drug resistance genes -- Novel mutation -- Mutation frequency; Communicable diseases -- Periodicals; Public health -- Periodicals; Epidemiology -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Medical microbiology -- Periodicals; 614.4
• DOI: 10.1016/j.jiph.2017.04.008
• Languages: English
• ISSNs: 1876-0341

Abstract

Abstract: Drug-resistant tuberculosis (DR-TB), especially multidrug-resistant tuberculosis (MDR-TB), is a serious medical and societal problem in China. The purpose of this study was to evaluate the mutation characteristics of drug-resistant Mycobacterium tuberculosis ( M. tuberculosis ) isolates in Yunnan, China. Drug susceptibility testing (DST) was performed in 523 clinical M. tuberculosis isolates. Six drug resistance genes ( katG, inhA, rpoB, rpsL, embB, and pncA ) were selected to screen for mutations. In total, 54 clinical M. tuberculosis strains were identified as drug-resistant by DST, including 18 single drug-resistant (SDR) strains and 36 multidrug-resistant (MDR) strains. Twenty-four types of mutations in five genes (excluding the inhA gene) were screened in forty-one strains. Six novel mutations were identified in this study, including three missense mutations (p.S302R in katG, p.D78G in embB, and p.M1I in pncA ), two frameshift mutations (408 ins A and 538-580 del in pncA ), and one mutation in a control region (−6 C>T located upstream of rpsL ). The mutation frequencies in the hotspot mutation regions in the katG, rpoB, rpsL, embB, and pncA genes were 92.5%, 44.4%, 54.2%, 52.6%, and 37.5%, respectively. The mutation spectra and frequencies seemed somewhat unique in the Yunnan DR-TB strains.