A dual-application poly (dl-lactic-co-glycolic) acid (PLGA)-chitosan composite scaffold for potential use in bone tissue engineering. Issue 16 (2nd November 2017)

• Record Type: Journal Article
• Authors: Boukari, Yamina; Qutachi, Omar; Scurr, David J.; Morris, Andrew P.; Doughty, Stephen W.; Billa, Nashiru
• Is Part Of: Journal of biomaterials science. Volume 28:Issue 16(2017)
• Page Start: 1966
• Page End: 1983
• Publication Date: 2017-11-02
• Subjects: Polymeric biomaterials -- controlled delivery -- poly (lactic-co-glycolic acid) (PLGA) -- microspheres -- protein delivery -- tissue engineering -- mechanical properties -- formulation; BMPs: bone morphogenetic proteins -- BSA: bovine serum albumin -- DCM: dichloromethane -- DMSO: dimethyl sulphoxide -- ECM: extracellular matrix -- FTIR: Fourier transform infrared -- hMSC: primary human mesenchymal stem cells -- PBS: phosphate-buffered saline -- PLGA: poly (lactic-co-glycolic acid) -- PVA: poly (vinyl alcohol) -- SDS: sodium dodecyl sulphate -- SEM: scanning electron microscopy -- TPP: sodium tripolyphosphate -- ToF-SIMS: time of flight secondary ion mass spectroscopy; Polymers -- Biocompatibility -- Periodicals; Biomedical materials -- Periodicals; 572.33
• DOI: 10.1080/09205063.2017.1364100
• Languages: English
• ISSNs: 0920-5063

Abstract

Abstract: The development of patient-friendly alternatives to bone-graft procedures is the driving force for new frontiers in bone tissue engineering. Poly (dl -lactic-co-glycolic acid) (PLGA) and chitosan are well-studied and easy-to-process polymers from which scaffolds can be fabricated. In this study, a novel dual-application scaffold system was formulated from porous PLGA and protein-loaded PLGA/chitosan microspheres. Physicochemical and in vitro protein release attributes were established. The therapeutic relevance, cytocompatibility with primary human mesenchymal stem cells (hMSCs) and osteogenic properties were tested. There was a significant reduction in burst release from the composite PLGA/chitosan microspheres compared with PLGA alone. Scaffolds sintered from porous microspheres at 37 °C were significantly stronger than the PLGA control, with compressive strengths of 0.846 ± 0.272 MPa and 0.406 ± 0.265 MPa, respectively ( p < 0.05). The formulation also sintered at 37 °C following injection through a needle, demonstrating its injectable potential. The scaffolds demonstrated cytocompatibility, with increased cell numbers observed over an 8-day study period. Von Kossa and immunostaining of the hMSC-scaffolds confirmed their osteogenic potential with the ability to sinter at 37 °C in situ.