Experimental design-based optimization of lipid nanocarrier as delivery system against Mycobacterium species: in vitro and in vivo evaluation. (3rd October 2017)

• Record Type: Journal Article
• Authors: Hussain, Afzal; Kumar Singh, Sandeep; Ranjan Prasad Verma, Priya; Singh, Neeru; Jalees Ahmad, Farhan
• Is Part Of: Pharmaceutical development and technology. Volume 22:Number 7(2017)
• Page Start: 910
• Page End: 927
• Publication Date: 2017-10-03
• Subjects: Mycobacterium species; electron microscopy studies -- SNEDDS formulations -- anti-tubercular study -- ex vivo CLSM; Drug delivery systems -- Periodicals; Pharmaceutical technology -- Periodicals; Drugs -- Administration -- Research -- Periodicals; Drug Delivery Systems -- Periodicals; Pharmaceutical Preparations -- Periodicals; Technology, Pharmaceutical -- Periodicals; 615
• DOI: 10.1080/10837450.2016.1212879
• Languages: English
• ISSNs: 1083-7450

Abstract

Abstract: The study aimed to optimize self-nanoemulsifying drug delivery system using experimental design using excipients holding innate anti -mycobacterium activities followed with characterizations for responses such as optical clarity ( Y 1 ), zone of inhibition (ZOI) against Mycobacterium smegmatis strains ( Y 2, Y 3 ), and globular size ( Y 4 ). The optimized formulations (OF1–OF3) were further characterized for responses and evaluated for zeta potential, minimum inhibition concentration (MIC) against non-pathogenic and tubercular strains, morphological (electron microscopy and atomic force microscopy), and confocal laser scanning microscopy (CLSM) studies. The desirability analysis suggested that the predicted values of the OF1 for the responses Y 1, Y 2, Y 3, and Y 4 were 0.137, 22.77 mm, 21.9 mm, and 191.11 nm, respectively. The morphological assessment confirmed the in vitro studies and established the inhibition mechanism as evidenced with oozing, ablation, and cell-wall fragmentation followed with cell disruption. The OF1, OF2, and OF3 showed an MIC value at 8.8 ± 0.56 mg/ml, 12.5 ± 0.22 mg/ml, and 15.0 ± 0.4 mg/ml, respectively, corroborating effectiveness against tubercular strain. CLSM studies revealed 75.1, 80.3, and 88.7% as an intense fluorescence intensity of OF1, OF2, and OF3, respectively, as compared with dye solution (∼53%). Conclusively, it can be inferred that the delivery of anti-tubercular drugs might be reassessed using excipients with inherent anti- mycobacterium activities.