Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus. Issue 16 (18th August 2017)

• Record Type: Journal Article
• Authors: Takehara, Kiyoto; Yano, Shuya; Tazawa, Hiroshi; Kishimoto, Hiroyuki; Narii, Nobuhiro; Mizuguchi, Hiroyuki; Urata, Yasuo; Kagawa, Shunsuke; Fujiwara, Toshiyoshi; Hoffman, Robert M.
• Is Part Of: Cell cycle. Volume 16:Issue 16(2017)
• Page Start: 1502
• Page End: 1508
• Publication Date: 2017-08-18
• Subjects: adenovirus -- killer-red -- melanoma -- nude mouse -- photodynamic therapy -- telomerase; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2016.1249548
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Melanoma is a highly recalcitrant cancer and transformative therapy is necessary for the cure of this disease. We recently developed a telomerase-dependent adenovirus containing the fluorescent protein Killer-Red. In the present report, we first determined the efficacy of Killer-Red adenovirus combined with laser irradiation on human melanoma cell lines in vitro . Cell viability of human melanoma cells was reduced in a dose-dependent and irradiation-time-dependent manner. We used an intradermal xenografted melanoma model in nude mice to determine efficacy of the Killer-Red adenovirus. Intratumoral injection of Killer-Red adenovirus, combined with laser irradiation, eradicated the melanoma indicating the potential of a new paradigm of cancer therapy.