Ultraviolet B irradiation increases keratin 1 and keratin 10 expressions in HaCaT keratinocytes via TRPV1 activation and ERK phosphorylation. Issue 9 (11th April 2017)

• Record Type: Journal Article
• Authors: Huang, Kuo‐Feng; Ma, Kuo‐Hsing; Liu, Pei‐Shan; Chen, Bo‐Wei; Chueh, Sheau‐Huei
• Is Part Of: Experimental dermatology. Volume 26:Issue 9(2017)
• Page Start: 832
• Page End: 835
• Publication Date: 2017-04-11
• Subjects: calcium influx -- epidermal growth factor -- keratinocyte differentiation; Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.13292
• Languages: English
• ISSNs: 0906-6705

Abstract

Abstract: In this study, we characterized the effect of ultraviolet B (UVB) irradiation with or without epidermal growth factor (EGF) on the regulation of keratinocyte differentiation under physiological concentration of Ca 2+ (1.8 mM). In addition, growth factor deprivation used to measure signal transduction and kinase phosphorylation in many studies is physiologically unreal. Therefore, 1% of serum was also included in all experiment. We found that UVB irradiation Ca 2+ dependently induced morphological differentiation and increased keratin 1 and 10 (K1/K10) expressions. Both were inhibited by treatment of cells with EGF. In quiescent cells, phosphorylation of ERK was stimulated by acute EGF treatment, while it rapidly desensitized in chronic EGF treatment or 1% serum exposure. UVB irradiation‐induced keratinocyte differentiation required Ca 2+ influx through TRPV1. Ca 2+ ‐dependent phosphorylation of ERK was responsible for the expression of K1/10. Cotreatment of cells with EGF during UVB irradiation inhibits the UVB irradiation‐induced differentiation by desensitizing ERK phosphorylation.