Adenosine Triphosphate stimulates differentiation and mineralization in human osteoblast‐like Saos‐2 cells. (18th May 2016)

• Record Type: Journal Article
• Authors: Cutarelli, Alessandro; Marini, Mario; Tancredi, Virginia; D'Arcangelo, Giovanna; Murdocca, Michela; Frank, Claudio; Tarantino, Umberto
• Is Part Of: Development growth and differentiation. Volume 58:Number 4(2016)
• Page Start: 400
• Page End: 408
• Publication Date: 2016-05-18
• Subjects: adenosine triphosphate -- cell differentiation -- minerals -- osteoblasts -- purinergic P2 -- receptors; Embryology -- Periodicals; Developmental biology -- Periodicals; Growth -- Periodicals; 574.3
• DOI: 10.1111/dgd.12288
• Languages: English
• ISSNs: 0012-1592

Abstract

Abstract : In the last years adenosine triphosphate (ATP) and subsequent purinergic system activation through P2 receptors were investigated highlighting their pivotal role in bone tissue biology. In osteoblasts ATP can regulate several activities like cell proliferation, cell death, cell differentiation and matrix mineralization. Since controversial results exist, in this study we analyzed the ATP effects on differentiation and mineralization in human osteoblast‐like Saos‐2 cells. We showed for the first time the altered functional activity of ATP receptors. Despite that, we found that ATP can reduce cell proliferation and stimulate osteogenic differentiation mainly in the early stages of in vitro maturation as evidenced by the enhanced expression of alkaline phosphatase (ALP), Runt‐related transcription factor 2 (Runx2) and Osteocalcin (OC) genes and by the increased ALP activity. Moreover, we found that ATP can affect mineralization in a biphasic manner, at low concentrations ATP always increases mineral deposition while at high concentrations it always reduces mineral deposition. In conclusion, we show the osteogenic effect of ATP on both early and late stage activities like differentiation and mineralization, for the first time in human osteoblastic cells. Abstract : In osteoblasts ATP can regulate several activities like cell proliferation, cell death, cell differentiation and matrix mineralization. ATP can increase alkaline phosphatase (ALP) activity in a dose dependent manner as well as it can reduce cell proliferation in human osteoblast‐like Saos‐2 cells. ATP can affect mineralization in a biphasic manner inducing an increase of Calcium deposits at lower concentrations and a reduction of mineral deposition at higher concentrations.