A novel sandwich-type immunosensor based on three-dimensional graphene–Au aerogels and quaternary chalcogenide nanocrystals for the detection of carcino embryonic antigen. (26th July 2017)

• Record Type: Journal Article
• Authors: Liu, Lei; Du, Ruifeng; Zhang, Yihe; Yu, Xuelian
• Is Part Of: New journal of chemistry. Volume 41:Number 17(2017)
• Page Start: 9008
• Page End: 9013
• Publication Date: 2017-07-26
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c7nj02253k
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : Cu2 ZnSnS4 nanocrystals were firstly used as electrocatalysts in H2 O2 reduction for ultrasensitive detection of carcino embryonic antigen. Abstract : In this work, a sandwich-type electrochemical immunosensor was fabricated for the detection of carcino embryonic antigen (CEA) with the novel label of quaternary chalcogenide nanocrystals – Cu2 ZnSnS4 . They illustrated efficient intrinsic peroxidase-like activity in the catalytic reduction of peroxide hydrogen, which was ascribed to the synergistic effect of elemental composition in quaternary chalcogenides. Meanwhile, because of a high specific surface and good biocompatibility, three-dimensional Au nanoparticle loaded graphene aerogels were selected as substrates to capture more antibodies (Ab1 ). Under optimal conditions, the immunosensor exhibited a wide linear range from 0.5 pg mL −1 to 20 ng mL −1 with a low detection limit of 0.15 pg mL −1 (S/N = 3) for CEA detection. In addition, high sensitivity, excellent selectivity, good reproducibility and stability were obtained for the immunosensor. All of these results show that the novel immunosensor has a promising application for the quantitative detection of CEA in clinical diagnosis.