Cytotoxic and antioxidant properties of phenolic compounds from Tagetes patula flower. (24th December 2014)

• Record Type: Journal Article
• Authors: Kashif, Muhammad; Bano, Samina; Naqvi, Sabira; Faizi, Shaheen; Lubna, ; Ahmed Mesaik, M.; Azeemi, Khawaja Shamsuddin; Farooq, Ahsana Dar
• Is Part Of: Pharmaceutical biology. Volume 53:Number 5(2015:May)
• Page Start: 672
• Page End: 681
• Publication Date: 2014-12-24
• Subjects: Antiproliferative -- free radical scavenger -- French marigold -- HeLa cell line -- patuletin; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.936471
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context : Tagetes patula Linn. (Asteraceae) (French Marigold) flowers are used by local practitioners for cancer treatment; however, it lacks scientific justification. Objective : Identification of bioactive compounds in T. patula flower for cytotoxic and growth inhibition in human cancer cell lines along with its antioxidant properties using chemical and cell based systems. Materials and methods : The T. patula flower methanol extract, its seven fractions, and three phenolic compounds including methyl protocatechuate (1 ), patuletin (2 ), and patulitrin (3 ) were evaluated using sulforhodamine-B assay against HeLa, HT-144, NCI-H460, MCF-7, PC-3, and SF-268 human cancer cell lines. In parallel, antioxidant activity was evaluated using chemical (DPPH ·, deoxyribose, and lipid peroxidation assays) and cell-based chemiluminescence systems (human neutrophils and mice macrophages). Results : The methanol extract and ethyl acetate insoluble fraction exhibited cytotoxic and growth inhibitory effects against HeLa in which2 exhibited highest cell growth inhibition (GI50 : 0.6 ± 0.1 µg/ml) and cytotoxicity (LC50 : 2.5 ± 0.1 µg/ml). It also scavenged LOO · (IC50 : 6.5 ± 0.7 µg/ml) and\def\newpage{\vfill \break } \nopagenumbers $ {\rm O}_{2^ - }^ \bullet $ \newpage \end (IC50 : 27.5 ± 1.3 μg/ml) in chemical systems and human neutrophils, respectively. However, 1 preferably scavenged H2 O2 –Cl − (IC50 : 0.5 ± 0.01 μg/ml) in mice macrophages. Discussion and conclusion : Compound2 from T. patula flower exhibited both growth inhibitory and cytotoxic properties while1 and3 were only growth inhibitory against HeLa.1 –3 also displayed antioxidant properties implying its probable role in growth inhibition/cytotoxic action. The present study provides scientific evidence for the use of T. patula flower in cancer treatment by traditional healer.