Flavonoid‐Modified Surfaces: Multifunctional Bioactive Biomaterials with Osteopromotive, Anti‐Inflammatory, and Anti‐Fibrotic Potential. Issue 4 (21st October 2014)

• Record Type: Journal Article
• Authors: Córdoba, Alba; Satué, María; Gómez‐Florit, Manuel; Hierro‐Oliva, Margarita; Petzold, Christiane; Lyngstadaas, Staale P.; González‐Martín, María Luisa; Monjo, Marta; Ramis, Joana M.
• Is Part Of: Advanced healthcare materials. Volume 4:Issue 4(2015)
• Page Start: 540
• Page End: 549
• Publication Date: 2014-10-21
• Subjects: bioactivity -- biomaterials -- flavonoids -- surface functionalization -- titanium; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201400587
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : Flavonoids are small polyphenolic molecules of natural origin with antioxidant, anti‐inflammatory, and antibacterial properties. Here, a bioactive surface based on the covalent immobilization of flavonoids taxifolin and quercitrin on titanium substrates is presented, using (3‐aminopropyl)triethoxysilane (APTES) as coupling agent. FTIR and XPS measurements confirm the grafting of the flavonoids to the surfaces. Using 2‐aminoethyl diphenylborinate (DPBA, a flavonoid‐specific dye), the modified surfaces are imaged by fluorescence microscopy. The bioactivity of the flavonoid‐modified surfaces is evaluated in vitro with human umbilical cord derived mesenchymal stem cells (hUC‐MSCs) and human gingival fibroblasts (HGFs) and compared to that of simple flavonoid coatings prepared by drop casting. Flavonoid‐modified surfaces show anti‐inflammatory and anti‐fibrotic potential on HGF. In addition, Ti surfaces covalently functionalized with flavonoids promote the differentiation of hUC‐MSCs to osteoblasts—enhancing the expression of osteogenic markers, increasing alkaline phosphatase activity and calcium deposition; while drop‐casted surfaces do not. These findings could have a high impact in the development of advanced implantable medical devices like bone implants. Given the broad range of bioactivities of flavonoid compounds, these surfaces are ready to be explored for other biomedical applications, e.g., as stent surface or tumor‐targeted functionalized nanoparticles for cardiovascular or cancer therapies. Abstract : Bioactive Ti surfaces functionalized with flavonoids taxifolin and quercitrin are successfully fabricated. These innovative, simple, cheap, and robust surfaces exhibit osteogenic, osteopromotive, anti‐inflammatory, and anti‐fibrotic properties with promising applications in implantable bone medical devices. Given the broad range of functionalities of flavonoid compounds (antioxidant, antibacterial, antiviral, etc.), these surfaces could be extended to other biomedical applications.