Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis. Issue 4 (2nd December 2014)

• Record Type: Journal Article
• Authors: Wang, Yiwei; Mithieux, Suzanne M.; Kong, Yvonne; Wang, Xue‐Qing; Chong, Cassandra; Fathi, Ali; Dehghani, Fariba; Panas, Eleni; Kemnitzer, John; Daniels, Robert; Kimble, Roy M.; Maitz, Peter K.; Li, Zhe; Weiss, Anthony S.
• Is Part Of: Advanced healthcare materials. Volume 4:Issue 4(2015)
• Page Start: 577
• Page End: 584
• Publication Date: 2014-12-02
• Subjects: dermal substitute -- integra -- skin -- tropoelastin -- wound healing; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201400571
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post‐surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization. Abstract : Human tropoelastin enhances wound vascularization. The effect is seen in large and small animal models. The figure shows live animal imaging and demonstrates increased blood vessel density 2 weeks post‐surgery in a mouse wound repair model. Tropoelastin has potential use as a facilitator of wound healing.