A label-free electrochemical aptasensor for the detection of kanamycin in milk. Issue 5 (28th January 2015)

• Record Type: Journal Article
• Authors: Zhou, Nandi; Luo, Jibao; Zhang, Juan; You, Yuanding; Tian, Yaping
• Is Part Of: Analytical methods. Volume 7:Issue 5(2015)
• Page Start: 1991
• Page End: 1996
• Publication Date: 2015-01-28
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c4ay02710h
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Kanamycin is detected based on the conformational change of the aptamer attached to the electrode surface and the corresponding SWV current change in [Fe(CN)6 ] 3−/4− solution. Abstract : Kanamycin is a widely used aminoglycoside antibiotic, and residual kanamycin in animal-derived food causes serious side effects. Herein, a simple aptamer-based assay is proposed for the label-free electrochemical detection of kanamycin. A 5′-SH-modified kanamycin-specific aptamer was self-assembled on the surface of a gold electrode through Au–S chemistry. Upon binding to kanamycin, the conformation of the aptamer changes and its coverage of the electrode surface increases, owing to the formation of a stem-loop structure in the complex. As a result, the electron transfer resistance between the solution species and the electrode is increased. Using [Fe(CN)6 ] 3−/4− as a probe, the square wave voltammetry (SWV) current response was utilised to determine the concentration of kanamycin. The detection range of the aptasensor was found to be 10–2000 nM, indicating a high sensitivity and a broad detection range. The specificity of detection was also determined. Furthermore, the assay was successfully employed in the detection of kanamycin in milk samples, with a similar response range and detection limit.