Higher expression of HSP70 and LOX-1 in the placental tissues of pre-eclampsia pregnancies. (30th April 2014)

• Record Type: Journal Article
• Authors: Sheikhi, Abdolkarim; Razdar, Sara; Rahmanpour, Haleh; Mousavinasab, Nouraddin; Ganji, Hossein Barzegar; Jafarzadeh, Abdollah
• Is Part Of: Clinical and experimental hypertension. Volume 37:Number 2(2015)
• Page Start: 128
• Page End: 135
• Publication Date: 2014-04-30
• Subjects: Co-expression -- HSP70 -- LOX-1 -- placenta -- pre-eclampsia; Hypertension -- Chemotherapy -- Periodicals; Hypotensive agents -- Periodicals; 616.132
• DOI: 10.3109/10641963.2014.913607
• Languages: English
• ISSNs: 1064-1963

Abstract

Abstract: Background : Pre-eclampsia, a hypertensive disorder of pregnancy is the main cause of fetal and maternal morbidity and mortality. Growing evidences suggest that placental oxidative stress involves in the pathogenesis of pre-eclampsia. The HSP70 is a novel marker of oxidative stress which binds with high avidity to LOX-1. The aim of this study was to evaluate the co-expression of HSP70 and LOX-1 in the placental tissues of normotensive and pre-eclamptic pregnancies. Materials and methods : The placental tissues were collected from 35 healthy women with normal pregnancies and 33 women with pre-eclampsia disorder. Expression of HSP70 and LOX-1 on the placental tissues was examined by using immunohistochemistry technique. The intensity of the molecules' expression was determined by semi-quantitative scoring. Results : The 34.3% and 37.1% of the healthy women did not express the HSP70 and LOX-1 on their placenta, respectively. All pre-eclamptic patients expressed HSP70 and LOX-1 with various scores. Indeed, the majority of the pre-eclamptic subjects had ≥3+ scores of the expression of HSP70 and LOX-1 on their placenta (60.6% and 66.7%, respectively). The percentage of the ≥3+ scores of the expression of HSP70 and LOX-1 was significantly higher in patients than those in healthy women ( p < 0.0001 for both). Similarly, the majority of the pre-eclamptic subjects had ≥3+ scores of the co-expression of HSP70 and LOX-1 molecules (57.6%) which was significantly higher in patients than those in control group ( p = 0.0001). Conclusions : These results showed higher expression of HSP70 and LOX-1 in the placental tissues of pre-eclampsia patients which represent the possible contribution of these molecules in the disease pathogenesis. Further studies need to clarify their role in the pathogenesis of preeclampsia disorder.