Enzyme‐Responsive Release of Doxorubicin from Monodisperse Dipeptide‐Based Nanocarriers for Highly Efficient Cancer Treatment In Vitro. (20th November 2014)

• Record Type: Journal Article
• Authors: Zhang, He; Fei, Jinbo; Yan, Xuehai; Wang, Anhe; Li, Junbai
• Is Part Of: Advanced functional materials. Volume 25:Number 8(2015)
• Page Start: 1193
• Page End: 1204
• Publication Date: 2014-11-20
• Subjects: dipeptide -- biodegradability -- nanocarriers -- enzyme‐responsiveness -- Schiff base; Materials -- Periodicals; Chemical vapor deposition -- Periodicals; 620.11
• DOI: 10.1002/adfm.201403119
• Languages: English
• ISSNs: 1616-301X

Abstract

Abstract : Small aldehyde molecule are demonstrated to induce cationic diphenylalanine to assemble into monodisperse enzyme‐responsive nanocarriers with high biocompatibility and excellent biodegradability. The formation of Schiff base covalent bond and accompanying π–π interaction of aromatic rings are found to be the mainly driving forces for the assembly of the nanocarriers. Interestingly, the nanocarriers show autofluorescence due to the n–π* transitions of C = N bonds, which lends them visually traceable property in living cells. Importantly, the nanocarriers can be taken in by cells and biodegraded in the cells. In addition, doxorubicin is easily loaded into the nanocarriers with high encapsulation amount, and its release can be triggered by tyrisin under physiological conditions. Noticeably, even at a very low drug concentration, the doxorubicin‐loaded nanocarriers still exhibit a much higher killing capacity of HeLa cells in vitro, compared to the equivalent‐dose free doxorubicin, indicating they have a great potential biomedical application. Abstract : Cationic diphenylalanine (CDP) can assemble into biocompatible and biodegradable nanocarriers through cross‐linkage of glutaraldehyde (GA). The nanocarriers can be biodegraded under the action of tyrisin. Importantly, after being loaded with doxorubicin (DOX) the nanocarriers also show a desired enzyme‐responsive property, and the release of DOX can be easily achieved in PBS (pH 7.2) with tyrisin.