The human oligodendrocyte proteome. Issue 23 (20th November 2013)

• Record Type: Journal Article
• Authors: Iwata, Keiko; Café‐Mendes, Cecilia C.; Schmitt, Andrea; Steiner, Johann; Manabe, Takayuki; Matsuzaki, Hideo; Falkai, Peter; Turck, Christoph W.; Martins‐de‐Souza, Daniel
• Is Part Of: Proteomics. Volume 13:Issue 23/24(2013:Dec.)
• Page Start: 3548
• Page End: 3553
• Publication Date: 2013-11-20
• Subjects: Biomedicine -- Brain -- MO3.13 -- Oligodendrocytes -- Multiple sclerosis -- Schizophrenia; Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201300201
• Languages: English
• ISSNs: 1615-9853

Abstract

Abstract : Myelination of the CNS is performed by oligodendrocytes (OLs), which have been implicated in brain disorders, such as multiple sclerosis and schizophrenia. We have used the human oligodendroglial cell line MO3.13 to establish an OL reference proteome database. Proteins were prefractionationated by SDS‐PAGE and after in‐gel digestion subjected to nanoflow LC‐MS analysis. Approximately 11 600 unique peptides were identified and, after stringent filtering, resulted in 2290 proteins representing nine distinct biological processes and various molecular classes and functions. OL‐specific proteins, such as myelin basic protein (MBP) and 2′, 3′‐cyclic nucleotide 3′‐phosphodiesterase (CNP), as well as other proteins involved in multiple sclerosis and schizophrenia were also identified and are discussed. Proteins of this dataset have also been classified according to their chromosomal origin for providing useful data to the Chromosome‐centric Human Proteome Project (C‐HPP). Given the importance of OLs in the etiology of demyelinating and oligodendrogial disorders, the MO3.13 proteome database is a valuable data resource. The MS proteomics data have been deposited to the ProteomeXchange with identifier PXD000263 (http://proteomecentral.proteomexchange.org/dataset/PXD000263 ).