Rai is a New Regulator of Neural Progenitor Migration and Glioblastoma Invasion123. (9th April 2012)

• Record Type: Journal Article
• Authors: Ortensi, Barbara; Osti, Daniela; Pellegatta, Serena; Pisati, Federica; Brescia, Paola; Fornasari, Lorenzo; Levi, Daniel; Gaetani, Paolo; Colombo, Piergiuseppe; Ferri, Anna; Nicolis, Silvia; Finocchiaro, Gaetano; Pelicci, Giuliana
• Is Part Of: Stem cells. Volume 30:Number 4(2012)
• Page Start: 817
• Page End: 832
• Publication Date: 2012-04-09
• Subjects: Glioblastoma -- Cancer stem cells -- Adult neural stem and progenitor cells -- Cell migration -- Invasion -- Shc; Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.1056
• Languages: English
• ISSNs: 1066-5099

Abstract

Abstract: The invasive nature of glioblastoma (GBM) is one important reason for treatment failure. GBM stem/progenitor cells retain the migratory ability of normal neural stem/progenitor cells and infiltrate the brain parenchyma. Here, we identify Rai (ShcC/N‐Shc), a member of the family of Shc‐like adaptor proteins, as a new regulator of migration of normal and cancer stem/progenitor cells. Rai is expressed in neurogenic areas of the brain and its knockdown impairs progenitor migration to the olfactory bulb. Its expression is retained in GBM stem/progenitor cells where it exerts the same promigratory activity. Rai silencing in cancer stem/progenitor cells isolated from different patients causes significant decrease in cell migration and invasion, both in vitro and in vivo, providing survival benefit. Rai depletion is associated with alteration of multiple‐signaling pathways, yet it always leads to reduced expression of proinvasive genes. STEM CELLS 2012;30:817–832