Intracerebral microdialysis coupled to LC–MS/MS for the determination tramadol and its major pharmacologically active metabolite O‐desmethyltramadol in rat brain microdialysates. Issue 8 (7th March 2017)

• Record Type: Journal Article
• Authors: Liu, Mingzhou; Wang, Peng; Yu, Xuming; Dong, Guicheng; Yue, Jiang
• Is Part Of: Drug testing and analysis. Volume 9:Issue 8(2017)
• Page Start: 1243
• Page End: 1250
• Publication Date: 2017-03-07
• Subjects: tramadol -- O‐desmethyltramadol -- microdialysis -- LC‐ESI‐MS/MS -- pharmacokinetics; Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.2157
• Languages: English
• ISSNs: 1942-7603

Abstract

Abstract : A rapid and sensitive method involving liquid chromatography electrospray tandem mass spectrometry (LC‐ESI‐MS/MS) coupled to an intracerebral microdialysis technique was developed for the determination and pharmacokinetic investigation of tramadol and its major active metabolite O ‐desmethyltramadol (ODT) in rat brain. The microdialysis samples were separated on a C18 column and eluted with a mobile phase of acetonitrile‐water‐formic acid (50:50:0.1; v/v/v ) at a flow rate of 0.3 mL/min. The ESI‐MS/MS spectra were performed in electrospray positive ion mode, and the analytes were detected by multiple reaction monitoring (MRM) of the transitions m/z [M + H] + 264.3 → 58.2 for tramadol, m/z [M + H] + 250.3 → 58.3 for ODT, and m/z [M + H] + 379.4 → 264.0 for ambroxol (internal standard; IS). The total run time was 4.0 min. A lower limit of quantitation (LLOQ) was achieved as 1 ng/mL for tramadol and 0.5 ng/mL for ODT, with excellent linearity over a concentration range of 1 ~ 500 ng/mL ( r > 0.99) for tramadol and 0.5 ~ 50 ng/mL for ODT ( r > 0.99), respectively. The proposed method was successfully applied to the pharmacokinetic studies of tramadol and ODT in rat brain. Copyright © 2017 John Wiley & Sons, Ltd. Abstract : A simple and sensitive LC‐ESI‐MS/MS coupled to Intracerebral microdialysis method coupled to an intracerebral microdialysis technique was developed for the simultaneous determination of tramadol and its major active metabolite O‐desmethyltramadol in rat brain. The proposed method was successfully applied to the pharmacokinetic studys of tramadol and ODT in rat brain.