A novel enzymatic method for synthesis of glycopeptides carrying natural eukaryotic N-glycans. Issue 65 (28th July 2017)

• Record Type: Journal Article
• Authors: Xu, Yangyang; Wu, Zhigang; Zhang, Peiru; Zhu, He; Zhu, Hailiang; Song, Qitao; Wang, Li; Wang, Faxing; Wang, Peng George; Cheng, Jiansong
• Is Part Of: Chemical communications. Volume 53:Issue 65(2017)
• Page Start: 9075
• Page End: 9077
• Publication Date: 2017-07-28
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c7cc04362g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Production of glycopeptides carrying complex-type N-glycans via three steps of enzymatic reactions. Abstract : A novel enzymatic approach was developed for facile production of glycopeptides carrying natural eukaryotic N-glycans. In this approach, peptides can be GlcNAcylated at one or two natural N-glycosylation sites via two-step enzymatic reactions catalyzed by an evolved N-glycosyltransferase (ApNGT Q469A ) and a glucosamine N-acetyltransferase (GlmA), respectively. The resulting GlcNAc-peptides were further modified by an endo -β-N-acetylglucosaminidase M mutant (EndoM N175Q ) to generate glycopeptides. In three steps of enzymatic catalysis, glycopeptides carrying complex-type N-glycans can be efficiently synthesized.