Hepcidin expression from monocyte of splenectomized and non-splenectomized patients with HbE-β-thalassemia. Issue 3 (April 2014)

• Record Type: Journal Article
• Authors: Pratummo, Kanita; Jetsrisuparb, Arunee; Fucharoen, Supan; Tripatara, Amporn
• Is Part Of: Hematology. Volume 19:Issue 3(2014)
• Page Start: 175
• Page End: 180
• Publication Date: 2014-04
• Subjects: Hepcidin -- Thalassemia -- Splenectomy -- Monocyte -- IL-6; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; 616.15005
• DOI: 10.1179/1607845413Y.0000000110
• Languages: English
• ISSNs: 1024-5332

Abstract

Abstract : Objective: Hepcidin is a key regulator of body iron homeostasis. The inflammatory cytokine interleukin (IL)-6 has been reported to upregulate expression of the hepcidin ( HAMP ) gene in monocytes. The purpose of this work was to determine HAMP expression at steady state in monocytes of splenectomized and non-splenectomized patients with HbE-β-thalassemia compared with normal controls. Methods: Levels of HAMP mRNA were measured using real-time reverse transcriptase polymerase chain reaction. Plasma IL-6, soluble transferrin receptor (sTfR), and ferritin levels were determined by enzyme-linked immunosorbent assay, and C-reactive protein (CRP) by nephelometry. Results: Levels of HAMP mRNA, CRP, IL-6, sTfR, and ferritin were significantly higher in both groups of patients with thalassemia than controls, but were not different between splenectomized and non-splenectomized patients. Monocyte HAMP mRNA content of patients with thalassemia correlated with plasma IL-6 and CRP levels. Discussion: Patients with HbE-β-thalassemia have persistent elevation of the plasma inflammatory cytokines, CRP, and IL-6, and the latter could be responsible (in part) to the induction of HAMP expression in monocytes of patients with HbE-β-thalassemia.