A Comparative Study of Cymbopogon citratus Volatile Oil by Conventional Method Versus Enzyme Pre-Treatment. Issue 3 (4th May 2017)

• Record Type: Journal Article
• Authors: Rashmi, ; Bhardwaj, Sapna; Gupta, P.K.
• Is Part Of: Journal of essential oil-bearing plants. Volume 20:Issue 3(2017)
• Page Start: 744
• Page End: 751
• Publication Date: 2017-05-04
• Subjects: Cymbopogon citratus -- volatile oil -- enzymatic pre-treatment -- GC-MS analysis; Essences and essential oils -- Periodicals; Aromatic plants -- Biotechnology -- Periodicals; Oilseed plants -- Biotechnology -- Periodicals; Aromatic plants -- Periodicals; Oilseed plants -- Periodicals; 661.80605
• DOI: 10.1080/0972060X.2017.1328289
• Languages: English
• ISSNs: 0972-060X

Abstract

Abstract: The objective of the study is to evaluate the impact of enzymatic pre-treatment on chemical composition of Cymbopogon citratus leaves volatile oil. Enzymatic pre-treatment has been considered as a novel and an effective way to release bounded compounds and increase overall yield. Lemongrass leaves with enzymatic pre-treatment with enzymes viz . β-galactosidase and cellulase yielded 0.82 % and 0.91 % volatile oil respectively. While, with mixture of enzymes (β-glactosidase and cellulase), the yield of volatile oil was 0.97 %. GC-MS analysis of enzymatic per-treated oil showed thirty three chemical constituents (99.5 %) with significant variation in area percentage of major constituents. However, in conventional hydro-distillation method, twenty two chemical constituents (72.4 %) were present. On comparison of enzyme aided extraction using mixture of enzymes showed the presence of some new chemical constituent's viz .1R α-pinene (0.43 %), α-phellendren (0.46 %), D-limonene (0.53 %), and citronellol (0.64 %) and a very significant increase in percentage of major components like α-citral, β-citral, geraniol, and β-Linalool.