Γ-Fe2O3@SiO2 core-shell structured nanoparticle: Fabrication via surface treatment and application for plasmid DNA purification. Issue 15 (15th October 2017)

• Record Type: Journal Article
• Authors: An, Gye Seok; Choi, Soo Wan; Chae, Dong Ho; Lee, Hyeon Seung; Kim, Hyeong-Jun; Kim, YooJin; Jung, Yeon-Gil; Choi, Sung-Churl
• Is Part Of: Ceramics international. Volume 43:Issue 15(2017)
• Page Start: 12888
• Page End: 12892
• Publication Date: 2017-10-15
• Subjects: Maghemite -- Surface treatment -- Core-shell structure -- Zeta potential -- Plasmid DNA purification; Ceramics -- Periodicals; Céramique industrielle -- Périodiques; Ceramics; Periodicals; Electronic journals; 666
• DOI: 10.1016/j.ceramint.2017.06.183
• Languages: English
• ISSNs: 0272-8842

Abstract

Abstract: Plasmid DNA was purified using core-shell γ-Fe2 O3 @SiO2 nanoparticles synthesized by a common sol-gel method utilizing tetraethylorthosilicate (TEOS) as a silica precursor. The dispersibility and TEOS binding affinity of γ-Fe2 O3 nanoparticles were enhanced by acid/base treatment due to the formation of additional surface hydroxyl groups prior to SiO2 deposition. Since SiO2 was coated under basic conditions in the presence of ammonia as a catalyst, base-treated γ-Fe2 O3 nanoparticles were more suited for this modification due to exhibiting a larger zeta potential and a lower saturation magnetization loss. The performance of the synthesized γ-Fe2 O3 @SiO2 nanoparticles with a 15-nm-thick SiO2 layer in the purification of plasmid DNA was compared to that of commercial Fe3 O4 -based magnetic beads. The used DNA samples exhibited similar purities, and all samples had similar theoretical plasmid DNA binding capacities. However, γ-Fe2 O3 @SiO2 nanoparticles exhibited a faster separation speed and a larger saturation magnetization of 100 emu/g compared to that of commercial Fe3 O4 -based magnetic beads (75 emu/g).