An Auto‐Inductive Cascade for the Optical Sensing of Thiols in Aqueous Media: Application in the Detection of a VX Nerve Agent Mimic. Issue 32 (3rd July 2017)

• Record Type: Journal Article
• Authors: Sun, Xiaolong; Anslyn, Eric V.
• Is Part Of: Angewandte Chemie international edition. Volume 56:Issue 32(2017)
• Page Start: 9522
• Page End: 9526
• Publication Date: 2017-07-03
• Subjects: auto-inductive cascades -- signal amplification -- thiol detection -- thiol-disulfide exchange -- VX nerve agents; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201704472
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: A new auto‐inductive protocol employs a Meldrum's‐acid‐based conjugate acceptor (1 ) as a latent source of thiol for signal amplification, as well as optical detection of thiols. The auto‐induction is initiated by a thiol‐disulfide exchange that leads to the generation of β‐mercaptoethanol, which in turn decouples the conjugate acceptor to release more thiols, resulting in a self‐propagating cycle that continues until all the conjugate acceptor is consumed. Using1 in a two‐step integrated protocol yields a rapid, sensitive, and precise diagnostic assay for the ultratrace quantitation of a thiophosphate nerve agent surrogate. Abstract : Thiol detection : Combining thiol‐disulfide exchange with decoupling of a conjugate acceptor (1 ), a new thiol auto‐inductive cascade was developed for signal amplification, as well as colorimetric and fluorometric detection of thiols. Using1 in a two‐step protocol yields a rapid assay for the ultratrace quantitation of a thiophosphate nerve agent surrogate at ppb levels.