An Auto‐Inductive Cascade for the Optical Sensing of Thiols in Aqueous Media: Application in the Detection of a VX Nerve Agent Mimic. (3rd July 2017)

• Record Type: Journal Article
• Authors: Sun, Xiaolong; Anslyn, Eric V.
• Is Part Of: Angewandte Chemie. Volume 129:Number 32(2017)
• Page Start: 9650
• Page End: 9654
• Publication Date: 2017-07-03
• Subjects: Autoinduktive Kaskaden -- Signalverstärkung -- Thiol-Disulfid-Austausch -- Thiolnachweis -- VX-Nervengift; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201704472
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: A new auto‐inductive protocol employs a Meldrum's‐acid‐based conjugate acceptor (1 ) as a latent source of thiol for signal amplification, as well as optical detection of thiols. The auto‐induction is initiated by a thiol‐disulfide exchange that leads to the generation of β‐mercaptoethanol, which in turn decouples the conjugate acceptor to release more thiols, resulting in a self‐propagating cycle that continues until all the conjugate acceptor is consumed. Using1 in a two‐step integrated protocol yields a rapid, sensitive, and precise diagnostic assay for the ultratrace quantitation of a thiophosphate nerve agent surrogate.