Highly Sensitive Molecularly Imprinted Sensor Based on Platinum Thin‐film Microelectrode for Detection of Chloramphenicol in Food Samples. Issue 8 (19th May 2017)

• Record Type: Journal Article
• Authors: Zhao, Xiaojuan; Zhang, Qimei; Chen, Haiguang; Liu, Gongliang; Bai, Weidong
• Is Part Of: Electroanalysis. Volume 29:Issue 8(2017)
• Page Start: 1918
• Page End: 1924
• Publication Date: 2017-05-19
• Subjects: Chloramphenicol -- Molecularly imprinted polymer -- Platinum thin-film microelectrode -- Electropolymerization -- Poly(o-phenylenediamine); Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201700164
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A highly sensitive and selective electrochemical biomimetic sensor was fabricated for fast detection of chloramphenicol (CAP) in honey and milk samples. Platinum thin‐film microelectrode (Pt TFME), which could provide unique electrochemical properties and achieve measurement using very limited solution volumes, was surface‐modified by electropolymerizing o ‐phenylenediamine. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the preparation process of CAP‐imprinted poly( o ‐phenylenediamine) film and rebinding ability of CAP into the imprinted cavities. The electrochemical properties of the sensor were further investigated with square wave voltammetry (SWV) by using K3 Fe(CN)6 as an electroactive probe. The current difference of oxidation peaks of K3 Fe(CN)6 had a good linear relationship with the concentration of CAP in the range of 0.9–10 nM. The detection limit was 0.39 nM based on the signal to noise ratio of 3. The developed sensor was successfully applied to determine CAP in honey and milk samples, and the result was in good agreement with that obtained by high performance liquid chromatography‐mass spectrometry (HPLC‐MS). The sensor showed high sensitivity and excellent selectivity to CAP in comparison to other structurally related and/or normally existing antibiotics, and demonstrated great promise for the rapid quantification of CAP in real food samples and field analysis. Abstract :