The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration. Issue 15 (30th March 2017)

• Record Type: Journal Article
• Authors: Zhukova, Yulia; Hiepen, Christian; Knaus, Petra; Osterland, Marc; Prohaska, Steffen; Dunlop, John W. C.; Fratzl, Peter; Skorb, Ekaterina V.
• Is Part Of: Advanced healthcare materials. Volume 6:Issue 15(2017)
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• Page End: n/a
• Publication Date: 2017-03-30
• Subjects: cell adhesion -- interface -- migration -- nanotopography -- titanium; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201601244
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : Surface structuring of titanium‐based implants is known to modulate the behavior of adherent cells, but the influence of different nanotopographies is poorly understood. The aim is to investigate preosteoblast proliferation, adhesion, morphology, and migration on surfaces with similar surface chemistry but distinct nanotopographical features. Sonochemical treatment and anodic oxidation are employed to fabricate disordered, mesoporous titania (TMS) and ordered titania nanotubular (TNT) topographies on titanium, respectively. Morphological evaluation reveals that cells are polygonal and well‐spread on TMS, but display an elongated, fibroblast‐like morphology on TNT surfaces, while they are much flatter on glass. Both nanostructured surfaces impair cell adhesion, but TMS is more favorable for cell growth due to its support of cell attachment and spreading in contrast to TNT. A quantitative wound healing assay in combination with live‐cell imaging reveals that cell migration on TMS surfaces has a more collective character than on other surfaces, probably due to a closer proximity between neighboring migrating cells on TMS. The results indicate distinctly different cell adhesion and migration on ordered and disordered titania nanotopographies, providing important information that can be used in optimizing titanium‐based scaffold design to foster bone tissue growth and repair while allowing for the encapsulation of drugs into porous titania layer. Abstract : Different nanotopologies are generated on the surface of titanium implants by ultrasound treatment (left) and by anodic oxidation (right). The structural differences influence morphology and migration behavior of preosteoblastic cells growing on these surfaces. A quantitative wound healing assay in combination with live‐cell imaging reveals more collective cell migration behavior on the disordered mesoporous surfaces generated by sonochemistry (left).