Chemical composition differentiation of Shen‐Shuai‐Ning granule between combined decoction and separated decoction using HPLC‐DAD‐ESI‐QTOF‐MS. (23rd March 2017)

• Record Type: Journal Article
• Authors: Liu, Jianli; Zhang, Yu; Hao, Yimeng; Zhao, Ying; Li, Yanjiao; Qin, Rulan; Lv, Chongning; Lu, Jincai
• Is Part Of: Biomedical chromatography. Volume 31:Number 9(2017:Sep.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2017-03-23
• Subjects: chemical composition identification -- combined decoction and separated decoction -- HPLC‐Dad‐ESI‐QTOF/MS -- Shen Shuai Ning; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3949
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Shen‐Shuai‐Ning (SSN) granule, a traditional Chinese medicine formula, is widely used in clinical practice for treating chronic renal failure. However, its detailed chemical profile is unknown. Here, HPLC‐ESI‐QTOF‐MS was employed for the systematic chemical analysis of SSN. A total of 52 compounds were identified and the characteristic ions of the compounds were described. Furthermore, chemical consistency between the combined decoction and the separated decoction of SSN was evaluated using HPLC‐DAD. A chemical comparison between two preparations of SSN granule (combined decoction and separated decoction of Coptides Rhizoma) indicated a significant difference in the content of many compounds, including salvianolic acid A, salvianolic acid B, berberine, palmatine and epiberberine. As a result, separated decoction of Coptides Rhizoma would lead to a significantly decrease in depsides in Salviae Miltiorrhizae Radix et Rhizoma and an increase in alkaloids in Coptidis Rhizoma.