The growing impact of lyophilized cell-free protein expression systems. Issue 4 (4th July 2017)

• Record Type: Journal Article
• Authors: Hunt, J. Porter; Yang, Seung Ook; Wilding, Kristen M.; Bundy, Bradley C.
• Is Part Of: Bioengineered. Volume 8:Issue 4(2017)
• Page Start: 325
• Page End: 330
• Publication Date: 2017-07-04
• Subjects: cell-free -- cfps -- freeze dry -- lyophilize -- onconase -- protein expression -- protein synthesis -- tx-tl; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2016.1241925
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not only overcome cold-storage limitations, but also enable stockpiling for on-demand synthesis and completely sterilize the protein synthesis platform. Recently reported high-yield synthesis of cytotoxic protein Onconase from lyophilized E. coli extract preparations demonstrates the utility of lyophilized cell-free protein expression and its potential for creating on-demand biotherapeutics, vaccines, biosensors, biocatalysts, and high throughput protein synthesis.