Down-regulation of liver-intestine cadherin enhances noscapine-induced apoptosis in human colon cancer cells. Issue 9 (2nd September 2017)

• Record Type: Journal Article
• Authors: Tian, Xia; Liu, Meng; Zhu, Qingxi; Tan, Jie; Liu, Weijie; Wang, Yanfen; Chen, Wei; Zou, Yanli; Cai, Yishan; Han, Zheng; Huang, Xiaodong
• Is Part Of: Expert review of anticancer therapy. Volume 17:Issue 9(2017)
• Page Start: 857
• Page End: 863
• Publication Date: 2017-09-02
• Subjects: Colon cancer -- Liver-intestine cadherin (CDH17) -- noscapine -- apoptosis -- Bcl-2; Cancer -- Treatment -- Periodicals; 616.99406
• DOI: 10.1080/14737140.2017.1344097
• Languages: English
• ISSNs: 1473-7140

Abstract

ABSTRACT: Background : The aim of the present study was to explore the signaling pathway of noscapine which induces apoptosis by blocking liver-intestine cadherin (CDH17) gene in colon cancer SW480 cells. Methods : Human colon cancer SW480 cells were transfected with CDH17 interference vector and treatment with 10 µmol/L noscapine. The proliferation and apoptosis of SW480 cells were detected by MTT assay and AnnexinV-FITC/PI flow cytometry kit (BD), respectively. Cell invasion were assessed by transwell assays. Apoptosis related proteins (Cyt-c, Bax, Bcl-2 and Bcl-xL) levels were evaluated by western blot. Results : Compared to the noscapine group, the proliferation was decreased significantly and the apoptosis was increased significantly in SW480 cells of the siCDH17+noscapine group. Cyt-c and Bax protein levels in siCDH17+noscapine group was higher than that of the noscapine group, but Bcl-2 and Bcl-xL protein levels in siCDH17+noscapine group were lower than that of the noscapine group. Moreover, up-expression of CDH17 inhibited the efficacy of noscapine-induced apoptosis in SW480 cells. Conclusions : We inferred that down-expression of extrinsic CDH17 gene can conspicuously promote apoptosis-inducing effects of noscapine on human colon cancer SW480 cells, which is a novel strategy to improve chemotherapeutic effects on colon cancer.