A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus. Issue 2 (3rd April 2017)

• Record Type: Journal Article
• Authors: Jiang, Mengting; Luo, Xiangwen; Zhang, Deyong; Zhou, Xuguo; Ouyang, Xian; Zhang, Zhanhong; Li, Fan; Zhang, Songbai; Liu, Yong
• Is Part Of: Canadian journal of plant pathology. Volume 39:Issue 2(2017)
• Page Start: 212
• Page End: 217
• Publication Date: 2017-04-03
• Subjects: detection -- pepper -- Pepper vein yellows virus -- PeVYV -- RT-LAMP -- RT-PCR; détection -- piment -- RT-LAMP -- RT-PCR -- Virus à veines jaunes du piment -- VVJPi; Plant diseases -- Canada -- Periodicals; Plant diseases -- Periodicals; 632.05
• DOI: 10.1080/07060661.2017.1316774
• Languages: English
• ISSNs: 0706-0661

Abstract

Abstract: Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62°C for 45 min and the whole reaction took less than 1 h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.